microarray plate scanner  (Thermo Fisher)

Journal: Trends in analytical chemistry : TRAC

Article Title: Ultraspecific analyte detection by direct kinetic fingerprinting of single molecules

doi: 10.1016/j.trac.2019.115764

Figure Lengend Snippet: Nucleic acid detection technologies and their analytical performance.

Article Snippet: Bio-Rad CFX96) Extraction kits, primers, fluorogenic probes, thermostable polymerase, dNTPs ddPCR [ 27 , 33 ] Yes Amplification-based zM to fM 99.99% Limited Droplet generator + droplet reader (e.g., Bio-Rad QX100), thermal cycler Extraction kits, droplet generation reagents, primers, fluorogenic probes, thermostable polymerase Illumina sequencing (TruSeq) [ 27 ] Yes Amplification-based zM to fM >99% High Illumina sequencer (e.g., MiSeq, HiSeq, NovaSeq), thermal cycler Extraction kits, library preparation kits, fluorescent dNTPs, thermostable polymerase, flow cells Microarray [ 27 , 31 ] Yes Amplification-free nM to mM 50–80% High Microarray plate scanner (e.g., AffyMetrix GeneChip HT) Extraction and PCR/labeling kits, microarray chips ADNA [ 22 ] Yes Amplification-free aM to fM Not available Not available Dark field microscope (e.g., Zeiss Axiovert 200M) Probe-conjugated nanoparticles, patterned lipid arrays NanoString [ 27 ] Yes Amplification-free fM to nM 90–95% High nCounter system (e.g., nCounter MAX ) Flow cells, capture probes, fluorescently barcoded affinity probes Simoa [ 24 ] Yes Amplification-free fM to pM >95% Limited Simoa analyzer (e.g., HD-1, SR-X) Probe-conjugated microparticles, droplet generation reagents, primary and secondary detection probes, enzymatic signal developing reagents Time gated FRET ligation [ 26 ] Yes Amplification-free pM to nM Not available Limited Fluorescence plate reader (e.g., ThermoFisher KRYPTOR) FRET probes, adaptor probes, DNA or RNA ligase SiMREPS [ 17 ] Yes Amplification-free fM to pM ≥99.99999% Limited TIRF microscope (e.g., Olympus IX83, Oxford NanoImager) Flow cells, capture probes, fluorescent probes Open in a separate window Nucleic acid detection technologies and their analytical performance.

Techniques: Amplification, Multiplexing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Sequencing, Microarray, Microscopy, Ligation, Fluorescence